HindIII and MseI in the Biomolecular Profile of Hemophilia in Cameroon: A Primer Study

Numerous studies are being carried out on polymorphisms within the genes coding for factors VIII and IX due to their clinical relevance in context of hereditary disorders. The quests polymorphism can be used screen haemophilia through an affected family. In Cameroon, very few gene have been conducted. Thus, this study was aimed at detecting HindIII SNP F8 MseI F9 by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RLFP) method determining involvement severity complications haemophilia. Fifty-five consented patients from Centre Hospitalier et Universitaire de Yaoundé (CHUY) were recruited a convenience sampling basis. Finger-prick blood collected spotted filter papers which DNA extracted then subjected PCR-RFLP. We able recruit 55 (37.16%) 148 registered CHUY until December 2018. average age 15 years. Of patients, 42 (76.36%) had type A 13 (23.64%) B haemophilia, 41 (74.55%) severe form, 10 (18.18%) moderate form 4 (7.27%) mild form). recruited, 38 (69.09%) already osteoarticular complication remaining 17 (30.91%) no complication. Fifty-three (96.36%) participants significant association found with hemophilia (P = 0.00). No 0.56) On other hand, (27.27%) diagnosed SNPs 0.89) or 0.68)